Measurement Of PH In Single Cell Based On Optical Fiber Nanosensor's Outside Illumination

In recent years,with the development of the life science research,people's research on organism also from organ,tissue to cell and subcell,the new test technology of chemical and biology have do a great contribution for the research of biology.Fiber optical fluorescence nanosensor have great advantage:litter volume,higher resolution,shorter response time,and less sample.As a result,the living cell could be detected with less damage,micromation, multi-parameter,real time and noninvasion.It's not only could be used for clinic diagnose, detection and protection for the living and working environment,and acquire the information from man-trap,but also have great application on the dynamic test of vivo biology cell with minimally invasive and real time.The p-2000 microelectrode puller were used for fabricate optical fiber nanoprobe,then coating the fiber with vacuum coating machine,finally, constructing a novel fluorescence nanosensor system with the coated optical fiber and fluorescein,and applied it to study the pH_i of single CA16 cell.The results showed that FIR was positively correlated to pH_i range from 6.2 to 7.9.Calibration curve equation was Y=-25.27826+4.48026X(r=0.99851,P＜0.0001),the pH_i is 7.21±0.05.