The Function Exploration Of Four Plant WRKY Transcription Fastors

Tran-acting factors are a group of proteins that dominate cellular processes by regulating the expression of downstream mark genes. The transcription factors WRKY protein superfamily are widely presented in plants. It is well known that a majority of the plant biological processes are modulated by WRKY transcriptional factor. Until now 72, 98, and 102 WRKY genes are found in the genomes of Arabidopsis thaliana. Columbia, Oryza sativa. Japonica, and Oryza sativa. Indica. We cloned the full-length sequences of OsWRKY08, OsWRKY72, AtWRKY45, and AtWRKY75 cDNA. Using molecular technology we found that the expression level of OsWRKY08 and OsWRKY72 were regulated spatially and induced by various stresses and plant hormones like other two genes AtWRKY45 and AtWRKY75 respectively. Further genetic analysis showed the stress resistances of OsWRKY08 and OsWRKY72 transgenic lines were improved. Furthermore, we found apical dominance of 35S:OsWRKY72 transgenic Arabidopsis thaliana was repressed and the transgenic plants had significantly more shoot branches than control plants. The phenotypes of 35S:AtWRKY75 and 35S:OsWRKY45, beside the repressed apical dominance in different levels, suggested that OsWRKY72 and its orthology AtWRKY75 played an important role in the auxin signaling pathway.