Hypoxia And Redox Reaction Modulate K_ATP Current In Rat Ventricular Myocytes

Aim:The objectives of this study were to investigate the possible regulatory mechanisms of redox agent and hypoxia.on the KATP currentMethods:The whole-cell,cell-attached and inside-out patch-clamp techniques were used to record KATP current in rat ventricular myocytes.Results:Hypoxia for 15 min increased KATP current while 1 mmol/l of reduced glutathione (GSH) could reverse the increased KATP current .Under normal circumstances, 1 mmol/l of oxidized glutathione (GSSG) increased KATP current while 1 mmol/l of reduced glutathione (GSH) could reverse the increased KATP channel current. To further corroborate the effect of redox agent on the KATP channel, we employed redox couple H2O2 / DTT which got results similar to the previous under normal circumstances. Both GSSG and hypoxia acting on KATP channels involve activation of the same signaling pathways.In other words,it suggest that oxidized glutathione (GSSG) or hypoxia increases KATP current through PKC,PKG, CaMK II pathways not mediated by PKA pathway.Conclusion:Redox agent and hypoxia acting on KATP channels involve activation of the same signaling pathways. In other words, Redox agent or hypoxia increases KATP current through PKC,PKG, CaMK II pathways not mediated by PKA pathway.