Study On The Chelate Of Amino Acid Metal And Dipeptide Metal

Amino acid and dipeptide are important biomolecule, which play a key role in the living body and also are the basic ligands in the living body. They often couple with metallic factors, take effect on many biology processes such as the enzyme-catalysis and biological membrane transportation etc. The organic metallic elements are absorbed by the transportation system of the ligand instead of the metallic transportation system. For example, the amino acid and the peptide chelate use the amino acid and the peptide absorption channel respectively. Though the transportation system of the amino-acid and the peptide, the chelate totally permeates the intestinal mucosa and get in the blood. Organic metallic elements are protected by the ligand so that they are not affected by the physical and chemical factors which are unfavour to the metallic absorption in gastrointestinal tract. The electric charges in the organic trace elements intramolecular tend to the neutrality, it can be dissolved and absorbed well in the pH enviroment in vivo, so it can be absorbed in mucous membrane of small intestine easily and then get into blood to satisfy all cells' needs, this raise the utilization rate of the trace-elements. Therefore, the amino acid,peptide metal chelates' research is of great significance. The thesis introduced the procedure of synthesizing L-aspartic acid metal complex and aspartame metal complex with the raw material of L-aspartic acid,aspartame and metal elements iron,zinc,autunite,coppe.The thesis investigated the effect of reation temperation, pH value, and material ratio on the chelating reation. To determine the L-aspartic acid zinc complex' chelation ratio by orthogonal experiment, we get the best conditions of reaction. On this basis, the thesis synthesized L-aspartic acid autunite complex and L-aspartic acid coppe complex.The thesis also introduced the procedure of chelate reation with the raw material of aspartame and zinc nitrate,bluestone and autunite nitrate. And utilize organic solvent separate and purify aspartame metal complex.As ferrous salt easily oxidized into three price molysite. In the reaction process, by adding antioxidants reduced iron powder to prevent the oxidation of the ferrous salt, accomplished the synthesis of L-aspartic acid/aspartame ferrous complex. In addition, we discussed complexometric titration process of ferrous complex.In this experiment, we utilized organic solvent ethanol, acetone sedimentation separation method to separate and purify metal chelates. In the mean time, the chelation ratio of L-aspartic acid/aspartame zinc,autunite,coppe,ferrous complex was determined by EDTA complexometric titration and the products' structure were analyzed and confirmed by IR spectrometry.