Study On The Extraction, Purification, Structure And Bioactivity Of The Polysaccharides From The Mycelia Of Phellinus Nigricans

The mycelia polysaccharides(PNW)were extracted from the mycelia of Phellinus nigricans using boiling water. The extracted condition of PNW was optimized by orthogonal experiment. The optimum condition was that the temperature 100℃, four times, two hours per times and the diffusion ratio 1:30.GC analysis indicated that the PNW was composed of Man and Glc with the molar ratio of 2.68: 1.00.PNW1 was purified from PNW using a combination of techniques, such as: ethanol fractional precipitation, deproteinization, DEAE cellulose column chromatography and Sepharose CL-6B column chromatography. HPLC analysis manifested that PNW1 was uniform fraction and the molecular weight was about 33 thousand Dalton. GC analysis indicated that the PNW1 was composed of Gal, Man, Glc, Ara and Xyl with the molar ratio of 8.77:6.44:3.26:1.00:1.35.The chemical and instrumental analysis methods such as : Partial acid hydrolysis, Periodate oxidation, Smith degradation, IR, GC, GC/MS and ¹³C-NMR were used on the structure analysis of PNW1.Then the repeated structural unit of PNW1 was determined.Initially investigated the effects on mouse leukomonocyte proliferation in vitro by PNW and PNW1. The results were that both PNW and PNW1 had significant effects on leukomonocyte proliferation and can promote the effects on the leukomonocyte proliferation stimulated by Con A or LPS. The effects of PNW1 on leukomonocyte proliferation were better than PNW.