| Tumor is a type of common disease in human being whose death rate is next to cardiopathy. Oncogene plays an impotant part in tumorigenesis, and expresses at a high level in some kinds of tumor cells. Cripto-1, aslo called teratocarcinoma-derived growth factor-1, which was discovered in 1987 and was cloned from a human NTERA2/D1 embryonal carcinoma cDNA library. Cripto-1 has EGF-CFC motif as functional domain, which is a kind of membrane-associated protein weight about 20KD. Cripto-1 can not only interact with many cell-surface receptors, but also is reported to function as a co-receptor for TGF-βfamily members in some signaling pathways. There are many signal pathway that control the proliferation and apoptosis,which are also actived by Cripto-1. Cripto-1 is a multifunctional gene with many faces including gastrulation and germ layer formation, mammary gland development and milk protein expression as well as angiogenesis. Cripto-1 expresses at a high level in variety of tumor tissues but at a low level in formal tissues. Cripto-1 promotes tumorgenesis, malignancy and metastasis, which is deemed as a kind of oncogene.In this study, we cloned the EGF-CFC domain of Cripto-1 into prokaryotic expression plasmid, and expressed the protein in the E.coli BL21 induced by IPTG, and after purification and renaturation, the protein was used to prepare polyclonal antibody. Then we use the antibody to detect the expression of Cripto-1 in both human tumor and normal cell lines. This study established a bases for further investigation of the biological function of Cripto-1, and preparation of tumor diagnosis reagent target to Cripto-1.
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