Study On Physiological Biochemical Characteristics Of Chenopodium Glaucum Linn Under Salt Stress

Chenopodium glaucum L ,such as a local major halophyte was studied in this experiment, By watering seedlings, the fundamental ingredient (water content, metallic element, total nitrogen ,proline content ,total sugar and betaine content) in different parts of Chenopodium glaucum L under different salt stress(0mmol/L- Control,100mmol/L,200mmol/L,300mmol/L,400mmol/L,500mmol/L)were determined and investigated. The distribution characteristics and dynamic variations of the fundamental ingredient were analysised, to provide basis for further understanding the salt-resistant mechanisms and physiological ecology mechanisms and grasping the adaptation features of Chenopodium glaucum L , at the same time for the use of arid halophytes in Xinjiang also is of great scientific significance and o a wide range of applications value. the results are as follows:1. After the Chenopodium glaucum L were treated 30-day in different concentrations of salt stress, the water content of roots were higher than the control group, when the concentration of NaCl is 300 mmol/L, the water content was highest; water content of stems and leaves are lower than control. water content was at its lowest level When NaCl concentration was 500 mmol/L. After stress 60 days, the water content of the roots, stems and leaves were less than control, and had a slow downward trend. After be stressed 90 days, the water content of the roots was lower than that of control, the water content of stems in 100 mmol/L was lower than that of control, leaves were higher than that of control. Leaves>stem> root. The results showed that by the stress of all time, the water content of different parts of Chenopodium glaucum L changed very little, that may be caused by Chenopodium glaucum L producing a response to specific environmental conditions.2. The content of Na+ droped first and elevates later with the increasing of concentration of salt stress, and mostly concentrated with the root, it is explained that the root has stronger ability to intercept the Na+, only very few part of Na+ may go through the transportation tissue to the aerial parts in the adult plant so that reduced interference effect on metabolism of leaf blade. K~+ content increased in low-salt concentration (100mmol/L-200mmol/L) and decline in high-salt content, which is in accordance with report of the literature. With the extension of salt stress time, the contents of all parts gradually declining. In general, in all stages of stress, under low concentration of (100 mmol/L-200mmol/L) of salt, Na~+/K~+ relatively low, the ratio is also higher with the salt concentration increased.3. After stress 30, 60 days, the total nitrogen content in different parts of the samples showed that increased in the first decreased later, and at the maximum level in the concentration of 200 mmol/L; the changes trends of roots of the 90-day sample were same to above, in the stems and leaves, the contents were at the highest level in the concentration of 100mmol/L, after had a slight decrease, but not obvious. Leaves>stem>root.4. When NaCl concentration was in a certain scope, with the deepening degree of stress, Chenopodium glaucum L had obvious trend in the accumulation of material. With the increasing of concentration of salt stress, proline content of the sites are greater than that of control;With the increased concentration of salt stress, Betaine content increased in all parts of Chenopodium glaucum L, In general, content changes of roots and stems were obvious, Stem> root> leave; The total sugar content of roots at the 300mmol/L salt concentration were significantly higher than other concentration in various periods, changes of sugar content in stems and leaves were a relatively small, relatively stable, Root> stem> leaves. With the extension of salt stress time, at stressed 60 days, that is the medium-term growth of Chenopodium glaucum L, contents of proline, betaine and sugar were in the most level.5. In addition, salt stress in early stages(30 days), the composition contents changes in various parts of Chenopodium glaucum L had a turning point at 400 mmol/L , later K+, Na+ content, total nitrogen, total sugar content tended to stabilization, proline and betaine contents were significantly increased. In addition Chenopodium glaucum L in NaClconcentration of 400 mmol/L and 500 mmol/L has nearly shriveled, it was conjectured that Chenopodium glaucum L suffered severe salt jury in salt stress 30 days when NaCI concentration were 400 mmol/L and 500 mmol/L. Stress in 90 days, the maximum tolerance to salinity of Chenopodium glaucum L was 300 mmol/L, the maximum tolerance to salinity of Chenopodium glaucum L was 200 mmol/L at the end of growing period.