Differential Proteomics Of Penicillum Griseofulvum F208 Highly Producing Griseofulvin In Biosynthesis

18S rDNA genes of the high griseofulvin producing mutant Penicillium griseofulvum F208 and its original strain Penicillium griseofulvum 3.5190 were successfully isolated by PCR with the primers designed on the basis of the consecutive 18S rDNA sequences of fungi. Their sequences were submitted to GenBank (Accessoin No.EF608151 and EF607282). The phylogenetic tree based on 18S rDNA sequences showed that the evolutionary distance between the mutant F208 and the original strain 3.5190 was far away. The mutant F208 was closed to Penicillium urticae, but the original strain 3.5190 was closed to Penicillium commune. 18S rDNA could be used as a molecular maker to identify the difference between the original and the mutant highly producing griseofulvin.By groping sample preparation method and optimizing of various conditions, we founded a set of method of two-dimensional gel electrophoresis for total proteins from Penicillium griseofulvum mycelia. And then we use technique of two-dimensional gel electrophoresis and mass Spectrometry to comparative analyze the differential expression of proteins from P.griseofulvum F208 during the course of fermentation. Comparing with sample cultured at 72h, five special protein spots increased in abundance were chosen among all discrepancy spots from sample cultured at 192h which was in antibiotic production phase, then to be analyzed by MS and Mascot search program. The two special spots were successfully identified, the F208-3 spot is serine hydroxymethyl transferase, and the F208-4 spot is S-adenosylmethionine synthetase. Finally, functions of the two proteins were analyzed at the course of biosynthesis of griseofulvin.