Synthesis Of Biodiesel Catalyzed By Penicillium Expansum Lipase

In this article, a kind of low-cost lipase——Penicillium expansum TS414 lipase waschose as catalyst, and the immobilization of lipase, reaction systems and processes of biodiesel production were investigated. The main results were as follows:The immobilization of P. expansum TS414 lipase on macroporous resins by absorpsion was studied. Under optimal conditions: AB-8 as absorbent resin, mass ratio of AB-8 to lipase 1:1, pH value of phosphate buffer solution 9. 0 and adsorption time 6 h, the maximum immobilized lipase activity was obtained.The transesterification between soybean oil and alcohol by immobilized P. expansum TS414 lipase catalyst under different systems was investigated. The results showed that the immobilized lipase displayed low catalytic activity using methyl acetate or ethyl acetate as acyl acceptor; the maximum conversion could reach 92. 6% using ethanol as acyl acceptor under solvent-free condition, and over 88% of conversion was obtained when methanol was used as acyl acceptor in t-butanol. The stability of the immobilized lipase was excellent. The conversion rate remained over 83% under solvent-free condition and had not decreased in t-butanol system after the enzyme was reused for 8 times.The conditions for enzymatic transesterification of waste cooking oil were optimized by uniform design test in solvent-free system and t-butanol system. The conversion rate optimized by uniform design test could reach 88.1%, 82.7% respectively, and the immobilized lipase also displayed good stability in the two systems.