The Improvement Of Strain For Producing 10-HDA And The Control Of Fermentation

10-Hydroxy-2-Decenoic Acid was a type of unsaturated fatty acid, which had been only found in the royal jelly by now. 10-HDA showed remarkable biologic activity against bacterium, ulceration, cancer, tumour. Especially, 10-HDA could enhance the ability of immunity, and reduce the fat of blood. As a result, 10-HDA had skyscraping value in the domain of medicament and health protection. So, it had attracted more and more attention. This paper mainly studied many aspects, such as, the improvement of the produetivity of starting strain QYW522 with mutation breeding, the research of shaking flask condition and 50L fermentor respectively.Based on studies and comparisons, we used UV mutation method, UV and LiCl mutation method, mitrous acid mutation method and microwave irradiation method to treat with the starting strain. At last, we chose UV and LiCl mutation as the main method to induce the protoplast of QYW522. Because we could obtain higher positive mutation ratio.The paper studtied the effect factors for preparing the protoplasts of QYW522. The best conditions for preparing protoplasts were confirmed. The optimal combine enzyme system contained 1% lywallzyme, 1.5% snailase, 1% fibrin enzyme. The best osmotic stabilizer was 0.8mol/L MgSO4. The experiment used mycelium aged 30 hours as operated material. The greatest temperature and time were 33℃and three hours respectively.According to folium screening, 73 strains were obtained from regeneration culture medium of high osmotic. At last, 8 strains that had higher improvement ratio were chose to carry through next screening. The paper used the shaking flask assay to filtrate the strains that had the better ability of producting 10-HDA. The content of 10-HDA was quantified by high pressure liquid chromatography (HPLC). Finally, compared with the starting strain, a special strain Uv90s-29 was screened, its content of 10-HDA in the fementation liquid had greatly improved.The thesis observed appearance character of Uv90s-29, according to handbook of fungi identification and other reference book , this strain belong to Fungi Imperfecti, Moniliales, Moniliaceae, Aspergillase, Aspergillus Mich.ex Fr, A.Flavus-oryzae, A.Effusus Tiraboschi.Through the single factor and the orthogonal test, the paper investigated the fermentation conditions of shaking flask, the result showed that the carbon resource was the most important factor, followed by nitrogen, and finally the salts, the best culturing conditions were confirm. The best amount ofinoculation was 10 percent, the optimal strain aged 30 hours, the initial pH was 6.0, the temperature was 30℃, the speed of shaking flask was 220 r/min, the greatest time for producing 10-HDA was 72 hours.On the basis of conditions for shaking flask, the paper also did some research on 50L fermentor experiment, three groups of experiments were carried out. The maximum output of 10-HDA was 29.5342μg/mL, the changes of different parameters during the fementation process were analyzed.