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Acid Tolerance Scanning And Biological Chrictrestic Study On A Iactobacillus

Posted on:2009-09-23Degree:MasterType:Thesis
Country:ChinaCandidate:X JinFull Text:PDF
GTID:2120360245465951Subject:Fermentation engineering
Abstract/Summary:PDF Full Text Request
With improvement of people living standard, more foods were intaked. Simultaneously It was showed that cerebrovascular and heart disease, such as artherosclerosis (AS), coronary artery disease (CAD), cerebral apoplexy were more universal and was first killer for human health. Lactic acid bacteria are one of major microbial population in intestinal tract of animal and human and closely related to physiological function of boty. Lots of experiments showed that LAB has functions on Cholesterol removal ability in vivo and vitro.Koumiss was traditional fermented milk with more medical care function. So some exprements were conducted on 9 samples from Zhenlan, Abaka county, inner mongola.Biologicial characteristics and microbiological compositions of 11 samples of koumiss from Inner Mongolia were determined. 11 strains of lactibucilli were obtaind by traditional physiological and biochemical isolation and identification method, in which 2 strains was L. acidophilus and 1 strain was L. helveticus. Furthermore, one strain with high acid tolaronce and bile-acid resistance characteristic were scanned from thease strains. And that the cholesterol removal ability was conducted in vitro. The ruslts showed that the counts of lactic acid bacteria were 2.18×108 in a sample kumiss, as for the other 10 samples the counts of lactic acid bacteria were 2.49×108~2.98×109 cfu/mL. The counts of yeast were 2.60×106~1.63×108cfu/mL. Experement of acid tolerance and bile release property of this strain showed that rate of bile release by this strain was 35.2%. Namely, this strain can tolerate lower pH (2.5), high bile-acid resistance property and cholesterol removal ability.
Keywords/Search Tags:Koumiss, Microbial Composition, Lactic acid bacteria, Acid tolaronce, Bile-acid resistance, Cholesterol removal ability
PDF Full Text Request
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