Proteome Comparative Analysis Of The Seedling Of Cry1Cry2 Mutant And Columbia Wild-type-4 Of Arabidopsis Thaliana

Plant functional genomics is develping very fast in this century. However, the information of gene express on the transcription level still can hardly comfirm the gene's function. Because the direct execute factor of life is protein other than the gene. Moreover, in the process of transcription and translation, the protein varies in different organism, different tissue even different period. So, proteomics witch was a leading technology for large-scale analysis of protein expression and function has become a major field of functional geomics. The technology of proteomics required to separate large numbers of proteins, to identify them, and to study their modifications are by no means straight forward. Currently the only separation technology which provides highly purified proteins separated in a simple parallel process is 2-Delectrophoresis. In proteome projects, which aim to identify and characterize all proteins expressed by an organism or tissue, the identification of proteins plays a central role.Plant growth and development are regulated in response to various environmental signals, and light is particularly important in this regard as an environmental cue during plant development.To monitor the light environment, plants have evolved a series of photoreceptors.Phototropins are the most recently characterized group of plant photoreceptors. On the basis of molecular genetic studies in Arabidopsis, it is clear now that there are two types of blue light receptors in plants: cryptochromes and phototropins.These phototropins regulates Phototropism, Photomorphogenesis, and Photoperiodic Flowering in plants.The proteins 2-D maps of Arabidopsis wild typ (Col-4), blue light receptor double mutant (cry1cry2) were obtained by optimizing crucial factors and procedures such as sample treatment, electrophoresis parameter, and gel concentration. The results revealed that adapting lysis solution to treating sample, choosing moderate amount of sample, using pre-cast immobilized linear pH gradient pH 3~10 24cm dry strips for the first dimensional electrophoresis, 12% separation gel were used to the SDS-PAGE electrophoresis. Fifty one protein spots, which were differentially expressed in the cry1cry2 and col-4, were identified by the method: MALDI-TOF-TOF-MS peptide fingerprint analysis of the protein spots and protein database searching. Thirty eight of them were identified succesfully, including some transtriptional factors,binding proteins,carboxylase,oxygenase,defense and stress-related proteins and photosynthetic proteins.These proteins plays an important role in the Arabidopsis cryptochromes of mediating Plant Photomorphogenesis.And shows Multiple aspects of development are regulated the photoreceptors phytochromes (PHY), crytochromes (CRY), or phototropins (PHOT) acting alone or in combination with each other.