Proteomics Of Cerebral Ganglions In The Mollusks Of Three Species

Notarcus leachii cirrosus Stimpson (NLCS), Pleurobranchaea novaezealandiae Cheseman (PNC) and Achatina fulica Férussac (AFF) are all mollusk animals belonging to gastropod class, and the composition and distribution of their central nerve systems (CNS) are relatively similar. In recent 10 years, many outstanding achievements in neurobiology have been obtained use CNS of mollusk animals as research material.In this project, the cerebral ganglion (CG) from NLCS and PNC which belongs to the same subclass with Aplysia had been chosen as materials to launch proteomics and related functional protein research. Furthemore, AFF had also been chosen as experimental materials to research the diferential proteome of CG in AFF contaminated by cadmium ion, trying to screen out some biomarker proteins induced by cadmium ion, intended to be used to monitor the heavy metal contamination of soil. The establishment of analytical techniques and research findings revealed could help to clarify and correct understanding living ehavior and regulatory mechanism of nerve systems in animals including human. Thus, this project shows highly scientific significance and worth for research.Firstly, the CG from NLCS were anatomized and separated, then dissolved by lysis. After the centrifugation, protein samples could be prepared. IPG strip was selected in the isoelectric focusing to a two-dimensional electrophoresis (2-DE) to obtain to protein patterns of NLCS CG. This project used peptide mass fingerprint (PMF) technology to identify the whole protein group, and the CG molecular anatomy atlas was initially established. Some proteins which were important to maintain the cell's physiological function were well identified, such as transcriptional regulator and peptidase.Secondly, the author compared the 2DE patterns of CG between NLCS and PNC treated with the same experimental condition, discovered 11 differential protein spots, and then identified these points one by one. Through the analysis of proteins found only in the PNC CG which had the higher matching rate, appeared in the beta subunit of ATP synthase, suggestted that the CG in PNC has its unique physiological and biological functions related protein.Having been induced with cadmium ion through the food chain, the CG of AFF was analysized by differential proteomics technology to study its expression of differential proteins. 29 differential protein spots were discovered and identified by PMF technology after the comparison of the 2DE pattarns. Some of the differential proteins produced by the CNS were protease htpX-like protein, ABC (ATP-binding cassette) transporter, and ABC transporter substrate-binding protein related to the CG cell's resisting capacity to cadmium ion. The analytic technology of proteome established in this paper is suitable for screening some biomarker proteins in animals'CNS to monitor r the cadmium contamination of soil.