Studies On Effects Of Vanadate And Vanadium Complex On Growth And Physiology Of Two Marine Microalgae

The effects of vanadate and vanadium complex on the growth and physiology of two marine microalgae (Platymonas helgolanidica,Pavlova viridis ) were studied systemically and comprehensively.Through experiments, we get results as follows:1. Through the experiment of the effects of Ammonium Metavanadate on the growth and physiology of two marine microalgae,we got the following conclusion:(1) NH₄VO₃ at lower concentration (0.05, 0.4, 3.2mg/L) had no significant effect on the growth of Platymonas helgolanidica but it enhanced soluble carbohydrate content and soluble protein content .It could also enhance the Chlorophyll content on the third day, but had no significant influence on Chlorophyll content on the sixth day. Meanwhile, it decreased the enzyme activity of SOD of Platymonas helgolanidica. While it had no remarkable effect on physiology of Pavlova viridis.(2) NH₄VO₃ at higher concentration (4.8, 6.4 mg/L for Platymonas helgolanidica, while 6.4, 25.6 mg/L for Pavlova viridis) seriously inhibited the growth of the two marine microalgae.While the tolerance to NH4VO3 stress of Pavlova viridis was higher than that of Platymonas helgolanidica. At the same time the soluble carbohydrate content and soluble protein content of Platymonas helgolanidica was enhanced while the Chlorophyll content and enzyme activity of SOD decreased. The soluble carbohdrate content, soluble protein content and Chlorophyll-a content of Pavlova viridis decreased while the enzyme activity of SOD enhanced.(3) NH₄VO₃ at lower concentration had no significant effect on the growth of the two marine microalgae under Fe-deficiency stress, while NH4VO3 at higher concentration seriously inhibited the growth of them.(4) POD isozyme zymograms of the two marine microalgae were very different. The Platymonas helgolanidica contained seven isozyme bands while the Pavlova viridis contained only one isozyme band. With the concentration increase of NH4VO3, two new bands (Rf =0.096, Rf =0.140) of POD isozyme were induced, at the same time two bands (Rf =0.063, Rf =0.471) lost, also the colour of the major band(Rf =0.452) at first became daker then became lighter subsequently.2. Through the experiment of the effects of vanadium complex on the growth and physiology of two marine microalgae,we got the following conclusion:(1) Vanadium complex at lower concentration had no significant influence on the growth of the two marine microalgae, while it seriously inhibited the growth of the two marine microalgae at higher concentration.(2) When the two marine microalgae were under Fe-deficiency stress, vanadium complex could accelerate the growth of them. The optimum dose of vanadium complex for Platymonas helgolanidica was 50mg/L,while that for the Pavlova viridis was 75mg/L.(3) Vanadium complex at certain concentration could enhance Chlorophyll content, soluble carbohydrate content and soluble protein content.