The Preparation And Applications Of Fluorescein Isocyanate Doped Nanoparticle And Its Preliminary Biological Security Study

The nanotechnology has been developed since 1980s and nano materials have a huge perspective in their application owing to the special properties. According to the advanced directions of the bionanotechnology, This dissertation consists of four charpters including: the synthesis of (Fluorescein Isocyanate,FI- TC)-core shell nanoparticles and the investigation on their properties and biological security; the research on the photocatalyzed degration of Ethidium Bromide( EB) by ZnO.In chapter one, the variety and preparation of nanoparticles used mainly in fields of biomedical will be introduced . Highlights of new findings with significance in application of nanomaterials in chemistry ,environment,materials, biology et al are viewd. We performed some researches based on the theme of nanotechnology.In chapter two, the synthesis of FITC-core shell nanoparticles and the properties as well as the bio-label using were investgated. The novel fluorescent label method based on core-shell fluorescent nanoparticles which possessing simple preparation, dispersancy, stability, signal magnification has been widely used in the field of biotechnology and chemistry. In this thesis we combined the advantages of FITC with core shell naonparticles technology. A fluorophore modified silica precursor is synthesized by the reaction of amino-reactive organic fluorphores ,FITC with APTEOS .then the sol-gel method is used to produce discrete fluorescent hybrid FITC-silica nanoparticles. And then we investigation the properties of nanoparticles such as dye leakage ,optical characters,fluorescent fluorescent stability and so on, meanwhile using the nanoparticles to bio-label.In chapter three, biological security of FITC-core shell nanoparticles have been studied. Ames test which using Salmonella typhimurium was to detect the FITC-core shell nanoparticles whether if have mutate effects. The toxicity of FITC-core shell nanoparticles to Hela cell was also carried on. They supported some evidence to the the biocompatibility of the core-shell nanoparticles.In chapter four ,with the industial ,algricultural and urban pollution increas- Ing, the environmental contaminate become more and more severe. Some reports show that photocatalytis has great potential in dealing with poisonous and pollute substances using semiconductor nanomaterials as photocatalyzer. In this part the degradation of EB using nano ZnO as a phto-catalyzer was investigated.Factors that concern with a photocatalysis reaction including pH, irradiation time, usage of ZnO nano particles and oxidant on degradation of EB were also investigated. The results showed that the degradation efficiency was satisfied when the photo-catalyzed reaction was carried out under optimal conditions, indicating that the method established in this article has great potential for practical application.