The Study Of CoQ₁₀ Structure And The Interaction Between CoQ₁₀ And Biomembrane Molecules

Coenzyme Q10 widely exists in each kind of histiocyte in particular in mitochondrion of the cardic muscle cell, is one kind of important active enzyme in cardic muscle cell, react as electron transfer system in breath-chain of mitochondrion, and is a important part in breath-chain, participates in ATP synthesis. It is also one kind of oxygen free radical elimination medicinal preparation. Its unusual metabolism is connected with many diseases, and artificial supplement of coenzyme Q10 can slowdown many illness. The structure of Coenzyme Q10 is related with its function, but there are few research reports about the relationships between its structure and its function, conformation in solution. Therefore, it is very important to study the structure of coenzyme Q10 in the molecular level to make the mitochondrion disease pathology known.In the foundation of predecessor study, we gain Coenzyme Q10 in pig cardicmuscle, and use many kinds of physics method to identify, then assemble it to the biomembrane. Next study the interaction between the Coenzyme Q10 and the biomembrane by LB, and investigate its conformation in solution, determine the position in the biomembrane by Microscope technology (AFM). The interaction and the conformation in solution obtained has the possibility to provide the clue for its position in the biomembrane, the using degree and so on.At present through the experimental study, we obtains the following research result:1. This research discovers that it is the most appropriate when the saponified time is 30min, the saponified temperature is 90℃. In the paper-chromatographic experiment, when benzene:ethanol (1: 2), the Rf value and the spot diameter are suitable, may obtain the good separation effect.2. After adding the Coenzyme Q10 in the PC (PE) molecular film, the change of phase is delayed. And this has provided the evidence for the conclusion of Thomas HauB etc. Namely coenzyme Q10 has not lied in molecular film, but located in the hydrophobic region, at the same time enters the hydrogen chain to the membrane. Adding the cholesterol molecular in the PC (PE) molecular film, we may find that the second phase change comparatively more obvious. Then adding coenzyme Q10, we can see the membrane pressure increases rapidly, and the phase-change flatten out. Simultaneously we observe that it is more regular that Coenzyme Q10 influence with PC to PE. But when we add the coenzyme Q10 molecular earlier, and then add the cholesterol molecular, we may see the same result, and PC molecular film is more stable to PE molecular film, this may have certain relationships with the molecular shape.3. We observe the structure of Coenzyme Q10 molecular by AFM. And we may extrapolate that the Coenzyme Q10 molecular is catenarian. Along with the coenzyme Q10 - ether solution dilution, coenzyme Q10 molecular presents 6 kind of topology in the mica surface. It is easier in the mica surface to form the laminated porous region.4. The interaction between coenzymes Q10 and other bio-molecular is very obvious:(1) The coenzyme Q10 molecular act with GM molecular, the surface presents island shape. When the GM1 molecular act with coenzyme Q10 molecular, the surface is very rough, but it is more smooth when the GM2, GM3 molecular act with coenzyme Q10 molecular, and there are many pores. (2) In the ordinary circumstances the cholesterol molecular can stabilize the cell membrane, but after adding the coenzyme Q10 molecular, the permeation of the cell membrane has been enhanced. (3) The membrane of PE(100uL)+CoQ10(100uL) is more smooth, but the PE membrane is very rough. And the membrane of PE(100uL) +CoQ10 (100uL) + Chol(100uL) is very complex, the shape of aggregates are various.(4) When the proportion is PE:CoQ10:PBS (20M:10M:5ml), the shape and size of PE-CoQ10 liposomes is uniform, and the diameter is about 140nm.The above results provide important information for the position and function in biomembrane, conformation in solution, and the usage degree etc. And enrich and develop the theory of the coenzyme Q10 structure and function, simultaneously accumulate the material for the coenzyme Q10 application.