The Study On Biological Function Of Four AP2/EREBP Transcription Factors

Plant cell has a very complex signal transfer mechanism. In biotic and abiotic stresses they activate a diverse set of physiological, metabolic and defense systems to survive and to sustain growth. Transcription factors play very important roles in regulated the growth, development, signal transduction and gene expression.AP2/EREBP is a group of transcription factors, special in plants. They are induced by biotic and abiotic stresses, and very important in stress-related transcriptions. We isolated four transcription factors ERF1/ERF2 and DREB3/DREB4, which from wheat (Xiao baimai) cDNA library, in drought stress. The four transcription factors are all never be reported before. The study we worked before showed that, ERF1/ERF2 was induced by biotic and abiotic stresses and DREB3/DREB4 were only induced by abiotic stresses. In this study, we wanted to identify the biological functions of the four genes, through the transgenic overexperssion plants.Progresses of this research are following:1. The analysis of the ERF1/ERF2 transgenic tobaccos in salt stress:The T0 ERF1/ERF2 transgenic tobacco lines were treated in salt stress for 50 days. The result showed that the ERF1/ERF2 improved the salt tolerance of transgenic tobaccos.2. The analysis of the ERF1/ERF2 transgenic tobaccos in pathogens stress: The T0 ERF1/ERF2 transgenic tobacco lines were inoculated Pseudomonas syringae pv. Tabaci at six to seven leaves old. The study indicated the ERF1/ERF2 Increased tolerance to infection by pathogen.3. The effect of the ERF2 to transgenic tobaccos stomas: The stomas of transgenic tobaccos closed quickly in both drought and ABA treatment.4. The analysis of the DREB3/DREB4 transgenic wheats in drought stress: The drought tolerance of the DREB3/DREB4 transgenic wheats were identified by control the water. DREB3/DREB4 changed the physiological and metabolic parameters in drought conditions, improved the transgenic wheat's tolerance.In this study, we analyzed the function of ERF1/ERF2 and DREB3/DREB4 in salt and pathogen stress. The results indicated ERF1/ERF2 was related to biotic and abiotic stress response, and DREB3/DREB4 could improve the transgenic wheat tolerance in drought.