Study On The Biodiversity Of Halophilic Bacteria From Two Salt Lakes In Xin-Jiang And Selection And Purification Of Halocin From Haloarchaeal

To understand the diversity of halophilic archaea and bacteria diversity and the extracellular hydrolytic enzymes diversity of Barkol lake and Manasi lake located in Xin-Jiang Region, 51 strains were isolated from the samples.Considering the 16S rRNA encoding gene sequence, the halophilic bacteria were identified as members of Planococcaceae, Bacillaceae, Staphylococcus, halomonadaceae, Salicolaceae, Pseudomonadacaeae, Halobacteriacea. Screening the bacteria led to all the strains to produce different hydrolytic enzymes: protease (15 strains), esterase (23 strain), amylase (8 strains)and no strain produce lipase and cellulase.Halocins are bacteriocin–like proteins or peptides produced by many species of the family Halobacteriaceae and are externalized into the environment, they are useful models for studying the gene expression regulation and protein structure/function in the haloarchaea. 23 haloarchaeal strains were used to study antagonistic interactions among them. The result showed that there were 22 strains produced halocins. The halocin AJ35 was chosen to be cultivated in liquid medium for 5 days, a halocin from strain AJ35 was purified 21.16 fold by TCA precipitation, Sephadex G-100 charomotography, DEAE Sepharose Fast Flow charomotography.The halocin was estimated to be 29.1~20.1 by SDS-PAGE.