Screening Of Coenzyme Q₁₀ Highly Producing Strains From Rhodobacter Sphaeroides

In this article, a breeding strategy combining a general mutagenesis is carried out to improve Coenzyme Q₁₀ yield of Rhodobacter Sphaeroides.To focus on the optimization of Rhodobacter Sphaeroides culture conditions, a systematic method is established to optimize the production of Coenzyme Q₁₀ and the biomass.The production of Coenzyme Q₁₀ of the strain in liquid state fermentation is 231 % higher than original culture conditions.To mutate Rhodobacter Sphaeroides by means of mutagenesis with diethyl sulfate(DES), ultraviolet ray(UV) and lithium chloride(LiCl) consecutively, 14 Coenzyme Q₁₀ highly producting mutants are screened from Rhodobacter Sphaeroides by mutagenses and high through screening, and Coenzyme Q₁₀ production of mutants named X9 in liquid state fermentation is 323% higher than parent strain.A simple method of extracting and determining Coenzyme Q₁₀ content of strain is obtained. A high through screening established is a rapidlly screening method. They could be efficient methods for quantitative analysis of fermentation production of Coenzyme Q₁₀. Finally, the inherited stability of the mutant strain is studied.