The Research Of Atpase's Rotation Catalysis Stochastic Transition Dynamics

Molecular motors are nanometer system which can transduce the chemical energy into mechanical work. Native molecular motors, such as kinesin, myosin, ATPase and so on, play an important role in the process of the intracellular transport, DNA and RNA duplication, mitosis, muscle contraction, composition of ATP, and so on. Molecular motors involve two species: linear molecular motors and rotary molecular motors. The ATPase as a kind of typical rotary molecular motor has received extensive interests for its special dynamical mechanics in numerous motors. They can synthesize ATP using a transmembrane proton potential established by electrochemical gradient or perform as a proton pump using the hydrolysis energy of ATP. The efficiency of the ATPase is approximately 100%. The novel and challenging problems in this field need to be further discussed in the view of biophysics.The work has been finished under the inspirit of the above-mentioned significance. First introduce the master equation into the research of the rotary molecular motor, in order to know clearly about the mechanism of the rotary molecular motor from the point view of ATPase's dynamics. The mainly scientific works are as follows : Chose the catalytic sites of oneβsubunit, to acquire the trace of theγsubunit which rotates around 3α3βsubunits and regarded it as one dimension movement. Put forward for the first time a stochastic hopping model which describes the rotary four-state molecular motor's dynamic action. And obtained the relationship between the probability distribution and ATP's concentration, and the change curves between the rotary angle velocity, diffusion coefficient and ATP's concentration, load force, and got a result which accords with the rotary molecular motor's biological mechanism. And explains several experimental phenomena qualitatively and quasi-quantitively from theory.