The Cloning, Localization And Functional Analysis Of AKIN11 Gene In Arabidopsis Thaliana

Sugar as a powerful signal molecular plays an essential role in every aspect of plant growth and development for energy resource as well as the regulation of the expression of many important genesIn order to investigate the gene function of AKIN11 in SnRK1 family, the cDNA was inserted the downstream of the 35S promoter in the plant expression vector pEGAD and then was transformed to Onion Epidermal cell by the particle bombardment. We found that the green fluorescent was focused on the nucleus.Then, the recombination vector was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens through the floral dip method. Two independent homozygous transgenic lines, T3 progenies were obtained by the Basta screening and RT-PCR method. Mutants have no distinguishable phenotype from that of the wild type in nature including the sucrose content of leaves, however, the starch content showed an increase in leaves of transgenic lines, especially in 12 h from the illumination. RT-PCR analyses found that the AKIN11 gene affected the expression of the AGPase, which is a key enzyme in the pathway of the starch synthesis. In contrast, there are no changes in expression of the FBPase,SPS,SUS and AMY3. The results indicated that the AKIN11 gene may be the positive regulation of ApS1 and play an important role in the regulation of the pathway of the starch accumulation and sugar metaboliztion in Arabidopsis thaliana.RT-PCR analysis of AKIN11 expression pattern tells us that it is expressed in most tissues, especially in flower while there is no significant change of expression in Arabidopsis growth and development.