Studies On Catalysis Of ADTZ To Its Substrates And The Catalysis Properties By Means Of Electrochemistry

Objective: Aflation B₁(AFB₁) is one of the most potent toxic and carcinogenic substances. Armillariella sp(E-20), that produce aflatoxin-detoxifizyme(ADTZ), has been screened out by our research institute, and recombinate ADTZ(rADTZ) has been get from the method of genetic cloning and recombinate expression. Earier research showed that ADTZ detoxify AFB₁ though hydrolysis reaction, following the oxidizing reaction. Oxidation is an important priming process. This paper uses electrochemical method to discuss the path of ADTZ catalyzing AFB₁, and the character of ADTZ.Method: Ion exchange chromatography(IEC) and hydrophobic interaction chromatography(HIC) were used to purify and prepare high activity rADTZ and ADTZ. Nanomaterial CNTs with carboxyl group immobilise rADTZ and ADTZ by means of cross-linked. Eletrochemical methods, Cyclic voltammetry(CV) and Different pulse voltammetry(DPV), were employed to detect the oxidoreduction signals of Aflation B₁(AFB₁), Sterigmatocystin(ST), Versicolorin A(VA), 2,3-Dihyopynan(DHP), Tetrahydrofuran(THF), Benzofunan(BF) and 8-methoxy-psoralen(MOP). HRP was employed to comfirm the production of H₂O₂, and the influence of O₂ on oxidoreduction signals during ADTZ catalyzing was inspected by bringing N₂ or O₂ to the system.Results:(1) AFB₁, ST, VA, DHP, THF, 8-MOP and BF were detected by rADTZ-SWNT modified electroed, among which AFB₁, ST, VA, DHP and THF all had redox signals while 8-MOP and BF had no significant signal.(2) The ADTZ-AFB₁ reaction mixture was detected with HRP-SWNT modified electrode and resμlts showed that H₂O₂ were produced.(3) Oxygen enhanced the redox signals of the ADTZ-AFB₁ reaction.(4) The resμlts showed oxidoreduction existing in the catalysis of rADTZ. The production of H₂O₂ and the enhancement of redox signals, under the influence of O₂, rADTZ was supposed to be a kind of mono-oxygenase.