Effect Of Dietary Calcium, Phosphorus And Calcium: Phosphorus Ration On The Development Of Bone And The Functiong Of Osteoblast Of The Rats In Growing Period

The development process of bone is affected by genetic factor and various kinds of environmental factors. There are many environmental factors, and one of them is nutriment. Furthermore, dietary calcium, phosphorus and calcium:phosphorus ratio could affect the development process of bone directly, and they may affect bone mineralization by changing the cytoactive of osteoblast.In this experiment, 70 male Wistar rats aged 30d were divided into 7 groups randomly according to their body weigh, 10 for each group was considered as one batch for test. Rats in 7 groups were raised on diets with different dietary calcium and phosphorus ( Vit D and other nutrients are suitable). In this experiment, dietary calcium, phosphorus and calcium: phosphorus ratio could affect bone mineralization, biochemical indicator in blood serum, and the mRNA expression of OCN and COLLI. One of the purposes of this experiment is to reveal the relationship of dietary calcium, phosphorus, calcium: phosphorus ratio and bone mineralization, and determine the best level of dietary calcium and phosphorus for bone mineralization, that could provid scientific evidence of suitable dietary calcium and phosphorus for rats. The other purposes is approaching the relationship of dietary calcium, phosphorus, calcium: phosphorus ratio and the cytoactive of osteoblast, that could provid theoretical foundation for far- research of the relationship of dietary calcium, phosphorus, calcium: phosphorus ratio and the development of bone.Obtaining two conclusions through the experiment: 1, In the same dietary calcium or phosphorus which had different Ca:P ratio, the body weight and bone of the rats in growing period growth better in the dietary Ca:P ratio of 1.2:1 than 0.4:1 and 4:1. In the same dietary calcium: phosphorus ratio, the body weight and bone of the rats in growing period growth better in the dietary Ca:P ratio of 1.2:1, even in the group of LL and HH, but the other groups in the dietary Ca:P ratio of 0.4:1 and 4:1 had significant deviation. Which suggest that dietary calcium or phosphorus could affect the body weight and bone of the rats, furthermore, the dietary calcium: phosphorus ratio may be more important. 2. Unsuitable dietary calcium, phosphorus and calcium: phosphorus ratio affect the cytoactive of osteoblast acutely, they could damage the functiong of osteoblast and induce functional disorder causing less expression of OCN and COLL I, which affect bone mineralization and growth retardation. The cytoactive of osteoblast is better in the group with 1.0 %Ca and 0.83% P.