Phenotypic Characterization Of Drosophila Tumor Suppressor Gene Scrib Mutant In Developing Larvae And Phosphorylation Studies Of Scrib

As one of the neoplastic tumor suppressor genes (nTSGs), Drosophila scrib plays a critical role in the regulation of cell polarity and proliferation. scrib1 is one lethal allele: whose homozygotes lose the ability to organize an epithelial monolayer and have overproliferated imaginal discs, dying as giant larvae after an extended third instar stage. To clarify the relationship between cell polarity and proliferation in scrib mutant, we used cell number counting and immunofluorescence staining to dynamically observe the morphgenesis of the developing scrib larvae, the disrupting course of the epithelial architecture and the change of the cell number in their wing disc. In addition, we cloned and expressed the truncated scrib DNA sequences in vitro, and then constructed GST fusion proteins. We hope we could identify the aPKC phosphorylation sites in Drosophila Scrib by in vitro kinase assay, in order to study the mechanism of scirb regulate the polarity and proliferation.