Study On The Bioactive Compounds From A Marine Sponge Associated Alcaligenes Feacalis A72

Sponge associated bacteria are important sources of natural bioactive compounds. In the previous research work, a Alcaligenes feacalis A72 was isolated from marine sponge Stelletta tenui, which was found to have multiple antimicrobial activity. And some literatures documented that bacteria of Alcaligenes sp. could generate a series of antibiotics.Three compounds were isolated from the culture of bacterium A72 by using thin-layer chromatography, gel exclusion chromatography, silica column chromatography, reverse phase HPLC etc. The structures of the 2 compounds were elucidated by study of the 1D-NMR and 2D-NMR and MS spectra.CompoundⅠ: Cyclo-(D)-Pro-(D)-PheCompoundⅡ: Cyclo-(D)-Pro-(D)-LeuCompoundⅢ: a fatty acid with at least 9 carbon atoms.The 3 compounds were screened for antimicrobial activities and in vitro anti-tumor activities. CompoundⅠshowed weak inhibition against staphylococcus aureus. CompoundⅢshowed cytotoxic activity against cell line SPC-A-1 with IC50 18.61μg/ml, which is better than 5-FU...