Mapping Of A Rice(Oryza Sativa L.) Fatty Acyl Reductase OsMS2 Gene And Its Function In Pollen Wall Development

The formation of pollen wall is important for the development of pollen, however, its mechanisms still remain unknown. We present here a comprehensive characterization of gene OsMS2 (MALE STERILITY 2) and demonstrate its biochemical function in the development of pollen wall. The osms2 mutant was obtained from 60Coγ-ray treatment. Genetic analysis indicated that the osms2 mutant male sterility phenotype was controlled by a single recessive nuclear locus. Using map-based cloning approach, the OsMS2 locus was mapped between the two InDel markers, SJ622 and CL7-4 on chromosome 3. The region delimited by these markerss was about 35kb. In this region, there was a gene homologous to Arabidopsis male-sterile gene MS2. The result of sequence analysis showed a deletion of one nucleotide in the eighth exon, which resulted in frame shifted mutation. We are carrying on completementary analysis of OsMS2.Observation of tissue section showed that osms2 microspore mother cells could undergo normal meiosis stage, dyads stage and tetrads stage of...