The Preliminary Study Of The Relationship Of Runx2 And Osterix In Regulating The Their Target Genes Expression

The formation and differentiation of the skeleton is a complicated process involved many factors. Transcription factors play a vital role in the developing process.Runx2 and Osterix are both key transcription factors which we have known.Runx2 and Osterix make their functions in the different stage of the skeleton.Runx2 can accelerate the differentiation of the mesenchymal stem cell into preosteoblast and osterix plays a key role in the differentiation of the preosteoblast into the mature osteoblast.But now we haven't known what the relationship of Runx2 and Osterix is in the differentiation of osteoblast and how they regulate the genes downstream.To determine the relationship of Runx2 and Osterix in the regulating their genes downstream. In C3H10T1/2 cell line we analyzed the firefly luciferase activities.In the cells which transfected with pcMV5- runx2 or pcDNA3.1(-)-osterix,we found the expression of pGL3-opn-luc or pGL3-alp-luc or pGL3-oc-luc increased. In the cells which co-transfected with pcMV5-runx2 pcDNA3.1(-)-osterix,we found they have the synergistic effect in the regulation of palp-luc or popn-luc,had obviously no the synergistic effect in the regulation of poc-luc.In the RT-PCR experiment ,when we transfected the cells with pcMV5-runx2 or pcDNA3.1(-)-osterix,only induce the expression of opn mRNA.When we contransfected the cells with pcMV5-runx2 pcDNA3.1(-)-osterix,they induced the expression of opn mRNA significantly.So in the C3H10T1/2 cell,overexpression of Runx2 and Osterix can induce the transcriptional regulation of opn and manifest the synergistic effect.