The Biological Property Of Porcine Adipose Mesenchymal Stem Cells And Effects Of ATRA On The Adipogenesis In Vitro

Adipose tissue has been recognized as an active organ that can store and release energy, maintain glucose homeostasis, and secrete hormones and cytokines. Because regulation of terminal differentiation of adipocyte precursors is an important factor in development of obesity and type II diabetes, there has been a growing emphasis on understanding the process of adipogenesis. Adipogenesis is a complex process including proliferation of precursor cells, their commitment to the adipogenic lineage, and terminal differentiation. Although the terminal differentiation of adipocytes is intensively studied and well described in the 3T3-L1/F442A murine cell lines and primary cells, they also have certain limitations. Because preadipocytes are unipotent, already committed cells, they cannot be used when it is important to investigate very early differentiation events, i.e., the recruitment of preadipocytes from multipotent precursors. So key information regarding the commitment of precursor cells to the adipogenic lineage is still lacking. Multipotent adult MSCs are isolated from adipose tissue but have also been isolated from other tissues (bone marrow and skeletal muscle). These cells, under the proper conditions, differentiate into adipocytes, chondrocytes, myocytes, osteoblasts, tenocytes, and neural cells. Several groups have provides evidence that a putative mesenchymal stem cell population exists within adipose tissue of human,murine,rabbit and others,with the potential to generate adipogenic, osteogenic,and myogenic cell lineages in a cell culture environment.The most important features of adipose tissue as a stem cell source might be the relative expendability of this tissue and the consequent ease with which it can be obtained in relatively large quantities with minimal risk.This degree of self-renewal is comparable with that shown for bone marrow-derived mesenchymal stem cells, whilst maintaining the capacity for multi-lineage differentiation. The promise of cell-based therapy may also be facilitated by the relative accessibility of these cells and more likely potential for repeat.The aim of this paper is to illuminate the biological property and adipogenic of the porcine AMSCs, so as to apply AMSCs to study adipogenesis and diseases therapy and bioactive compounds production in future, and subsequently to establish the porcine AMSCs. For above reasons, the isolation, culture, proliferation and growth characteristics, surface markers, and in vitro differentiation of piglets AMSCs were assayed. The results are as...