The Research On A New Electrochemiluminescent Reagent And Its Application To DNA Oxidation Injury Expression

The ECL behavior of phthalhydrazidylazoacetylacetone in all kinds of medium has been studied.The experimental results have indicated that phthalhydrazidylazoacetylacetone a occurs two steps'oxidation as luminol, but its potential of the oxidation is lower than luminol's. Phthalhydrazidylazoacetylacetone has good performance of ECL on the surface of ITO glass, and it can aviod the inference of ITO's luminescence. The ECL of phthalhydrazidylazoacetylacetone not only takes place in alkaline medium, but also in acid medium .The linear concentration range of phthalhydrazidylazoacetylacetone relative to the ECL intensity is from 1×10^-6 to 1.5×10^-5mol/L with the detection limit for 5.7×10^-7mol/L in acid medium.The guanine's effect to ECL has been studied.The quenching effect of guanine on the luminol's ECL has been found .The linear concentration range of guanine relative to the ECL intensity is from 6.6×10^-8 to 6.6×10^-5mol/L .Guanine can enhance the phthalhydrazidylazoacetylacetone's ECL in alkaline medium, and the linear concentration range of guanine relative to the ECL intensity is from 5×10^-7 to 3.0×10^-6mol/L.Guanine can quench the phthalhydrazidylazoacetylacetone's ECL in acid medium, and the linear concentration range of guanine relative to the ECL intensity is from 2.0×10^-6 to 1.0×10^-5 mol/L.The mechanism of phthalhydrazidylazoacetylacetone's ECL on the surface of ITO glass has been discussed according the experimental phenomenon.The phenomenon of ITO self-luminescence has also been studied.It has been found that the machanism of phthalhydrazidylazoacetylacetone on the surface of ITO in different medium is dissimilarity. The phenomenon of ITO self-luminescence may be caused by the interaction of the active oxygen element and ITO's surface.