A Preliminary Study On The Effects Of Overexpressed Nogo-C In PC12 Cells On Survival, Differentiation And Neurite Extension

Nogo has been identified as an important inhibitory factor which prevents axon regeneration after adult mammalian CNS trauma. Three different transcripts of nogo gene have been recognized by their alternative promoter usage and gene splicing, and these proteins, named Nogo-A. Nogo-B.and Nogo-C, share a highly conserved C-terminal domain but they lack a same N-terminal signal sequence and are predominantly localized to the ER. Their sequences show a detectable homology to the small family of reticulon (RTN) proteins (Nogo would be RTN4).Nogo-C is the smallest alternative splicing form of nogo gene. It is highly expressed in peripheral tissues, especially in skeletal muscle. There is also some expression in brain, particularly in adult Purkinje cells. The functions of Nogo-C in CNS are still unknown, although it has the axongrowth inhibitory domain------Nogo-66. Deletion of nogo gene suggests thatthe elimination of Nogo alone is not sufficient to induce extensive axon regeneration. Generation of Nogo-A/B/C Mutant Mice, in fact, are embryonic lethality of most homozygous mutants, and lack of enhanced regeneration...