The Applications Of Microsatellite And EST Markers In Genetic Analyses Of Two Cyprinid Fishes

Habitat fragmentation is the primary reason for loss of biodiversity. In this paper, five microsatellites were applied to genotyping over 300 individuals of redfin culter (Cultrichthys erythropterus) from four naturally isolated and three recent anthropogenic fragmented lakes in the middle and lower reaches of the Yangtze River, which had undergone natural fishery resource regression in past decades as a result of habitat fragmentation, reckless inning and over-fishing. A pronounced fine-scale (Fst=0.062, P<0.001) genetic structure was detected. Despite the high gene diversity (H>0.9), departures from Hardy-Weinberg equilibrium (HWE) were observed in almost all populations studied. In accordance with the theory of genetic erosion in habitat-fragmented populations, results in this study revealed that human-made habitat fragmentation, although had happened for up to five decades only, has already negatively affected the genetic variability of redfin culter populations, and produced significant genetic differentiation among seven populations. This study proved the power of microsatellite markers for elucidating the impact of recent habitat fragmentation on natural populations of a wild fish in the Yangtze River.The common carp (Cyprinus carpio L.) is one of the most important cultured fishes in China. EST (expressed sequence tags) markers, which derived from the expressed sequences of cDNA, are of high values in genome mapping and marker-assisted selection. We aim to develop EST markers for genome mapping and population genetics of common carp by data mining. 67 pairs of primers (HB1-67) were designed for common carp ESTs from the GenBank database. 49 of which can successfully amplify stable specific bands in genomic DNA. 12 polymorphic EST...