Study On Transformation Of CCoAOMT CDNA Of Boehmeria Nivea In Model Tobacco Variety WS38

To control the content of lignin, which constitutes principally to influence the quality of Ramie fiber, to reach to the ideal effect by conventional breeding or plantation measures always goes in vain. It proves that decreasing the content or altering the components of lignin, carrying out low lignin breeding via gene engineering technology seem to be a prospect field of the genetic improvement of Ramie.In this study, we constructed the full length expression vector of CCoAOMT (Caffeoyl-CoA 3-O-methyltransferase), and then transformed it into model tobacco variety WS38 via Agrobacterium tumefaciens mediated transformation. In order to find out whether CCoAOMT of Ramie has effects in the synthesis of lignin and can decrease the content of lignin or not. The main results of this study are as follows,(1) The 35S promoter controlling full length cDNA of CCoAOMT of Ramie expression recombinant (pWM101-BnCCoAOMT) has been constructed into vector pWM101 and transformed into model tobacco variety by co-cultivative methods.(2) The 35S promoter controlling anti-sense cDNA of Ramie CCoAOMT recombinant (pBI121-antiBnCCoAOMT) is constructed into vector pBI121 and transformed into model tobacco variety WS38.(3) Molecular analysis and a primary histology study reveal that the Ramie gene affects the growth of tobacco when it is expressing constantly in the transformed plantlets whenever it is expressed in a sense or antisense way. The transforms are usually growth a little bit slower than the controls. The lignin contant in vascular bundle of leafstalk seems reduced in antisense transforms by stain with safranin but there is no apperantly different in sense transforms with non-transformed WS38 as control.The results above provide some references of the further study of CCoAOMT of Ramie and its function in the metabolism of lignin. In addition, the study accumulates the experiences on the gene engineering of Ramie to reduce lignin contents or to improved components of lignin to favor fiber unglueing.