Observation Of Ultrafine Structure Of Mitochondrial ATP Synthase And DNA With Atomic Force Microscopy

As a novel tool in nanostructure of materials special in life science, atomic force microscope runs a key role in the life process, and it can get the surface topographies of the sample and much more about the physical and mechanic properties. Through controlling the stylus-sample force, atomic force microscope can detect the title interaction of different properties and analysis the mechanical relation between biologic samples and evaluate the related surface characters such as distribution of surface charge, magnetic domain, and the congregate state. And this study tried to construct the whole process in the atomic force microscope detection.Based on the reviews on the principle and application of atomic force microscope, we have tried to contribute the database including sample preparation, image analysis, and faults identification through enough experiments on base crystal, cell buffers, and tips pollution and damage. Then, have studied the ultrafine structure of the mitochondria and sub-mitochondria combined with Tans Electronic microscopy and optical microscopy. And have gotten the exact size of ATP synthase which is an important membrane protein in the inner membrane of mitochondria with atomic force microscope and LB techniques on mature purification techniques to the enzyme. These parameters may provide the key data for the foundation of physical model on the catalysis mechanism of ATP synthase. Then, mainly centralized on the original height and elasticity of DNA molecule in portrait with atomic force microscope and LB techniques, and have got exciting fruit.. The exploration of the compression elasticity in landscape orientation is rare, and the authors have not encountered the direct data until now. and we have reached the original height with Tapping Mode AFM, 2.3 ±0.1nm, which is Close to the theoretic value(2.1nm);Secondly, reach the compression elasticity in landscape orientation with contact mode AFM through the beautiful force curve, the Young's modulus value, 0.55±0.15GPa.At last, we ended the paper with the preview to the biological applications and problems of atomic force microscope.