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Sequence Analysis And Salinity Stress Studies About UDP-glucose Dehydrogenase Of Dunaliella Salina (DsUGD)-a Key Enzyme In The Polysaccharide Metabolism

Posted on:2007-01-21Degree:MasterType:Thesis
Country:ChinaCandidate:S J HeFull Text:PDF
GTID:2120360185493975Subject:Microbiology
Abstract/Summary:PDF Full Text Request
Former studies showed that polysaccharides as a buffer can keep microorganism out of the damage by the high salinity. Dunaliella salina is kind of unicellular green alga with extreme salt-tolerance. The relationship between polysaccharides and salt-tolerance are comparatively less studied. So the studies about the salina polysaccharides are useful to understand its' mechanism of salt-tolerance.UDP-glucose dehydrogenase converts UDP-glucose to UDP-glucuronate. This product is one of the most important precursor for polysaccharide. In our laboratory, we have first cloned this gene from Dunaliella salina (DsUGD), and found that the high salinity can induce the expression. In order to uncover the relationship between the salinity tolerance of the salina and the expression of DsUGD , and improve the other species' tolerance with salinity, we analyze the change of salina polysaccharide and protein of DsUGD when the Dunaliella Salina transformed from low salinity to the high salinity. The results are laid out as following:First, the change of polysaccharide amount after dealing with the salina cell with different salinity.The results show that the exopolysaccharide under 3.5mol/L NaCl is higher than the other two salinities. It gets to the highest amount after 2 hours, and then decrease as normal level 8 hours later; The inner polysaccharide doesn't chang with the time, but the amount under 3.5mol/L NaCl is obvious higher...
Keywords/Search Tags:Dunaliella salina, UDP-glucose Dehydrogenase, Polysaccharide, Expression study, Western blotting
PDF Full Text Request
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