Expression And Purification Of A Humanized Anti-CTLA4 ScFv (hS) And Its' Immunotoxin Fusion Protein (hSP): Prokaryotic E.coli & Eukaryotic Pichia Pastoris System

Cytotoxic T lymphocyte associated Ag-4 (CD152) is a structural homologue of the T cell co-stimulatory receptor CD28 and plays an inhibitory role in regulating T cell responses by interacting with the common ligands CD80/CD86 on APC. The CTLA4's membrane expression is restricted to activated T cells and some tumor cells. This Ag could be a good marker of T cell activation and a target for immunotherapy and therapy of tumor.Anti-CTLA4 single-chain Fv (scFv) with the specific binding activity to CD152 can be potentially used in activated T cell and tumor cell targeting both in vitro and in vivo. Anti-CTLA4 scFv fused with a certain functional molecule can specifically kill target cells, and thus provide a possible way to achieve specific immunosuppression or tumor target therapy.Humanized anti-CTLA4 scFv and its immunotoxin fusion protein have good prospect of applying in the biomedical research, immunodisease,...