Studies On Fluorescence Spectra Characteristics Of Phytoplankton Pigments

Monitoring phytoplankton classes and their abundance is a routine task in marine ecology research. With the frequent occurrences of red tide, there is an urgent need for rapid analysis methods that can provide quatitative informations. Three dimensional fluorescence spectrum, also termed as excitation-emission matrix(EEM) spectrum, gives total fingerprints informations in the whole range of wavelenghths. By using chemomatrics on extracting their pigments spectral features, EEM spectrum is often used for multicomponents analysis.This paper aims to study the characteristics of EEM spectra of eighteen phytoplankton species,they are Prorocentrum donghaiense,Prorocentrum minimum, Alexandrium tamarense , Alexandrium catenella , Alexandrium minitum , Gymnodinium sanguinem, Skeletonema costatum , Chaetoceros curvisetus , Chaetoceros minutissimus, Chaetoceros compressus, Chaetoceros tortissimus, Chaetoceros debilis, Rhizosolenia delicatula, Thalassiosira sp., Thalassiosira rotula, Ditylum brightwelli; Chlorella pyrenoidosa; Isochrysis galbana. Extracted the fluorescence spectra characteristics of phytoplankton pigments mentioned above, in order to identify typical phytoplankton in class or further detail levels. The main reseachment is as follows:1. The method of fluorescence excitation spectra remains the most specific to the variability of pigment composition to characterise phytoplankton. Fluorescence excitation spectra of culture samples were measured at the spectral range (excitation 400 to 660 nm, emission 680 nm). It is obvious that it is quite difficult to distinguish the species/class identification such as Alexandrium tamarense and Alexandrium catenella. But we can distinguish different genus;2.Spectral feature extraction methods were studied. The EEms were processed by...