Research On The Effect Of Serotonin On Sensation Afferent Function Of Gastrointestinal Tract

Objective: The purpose of this study was to explore the effect of Serotonin on sensation afferent function of gastrointestinal tract and the corresponding mechanism.Methods: 1.Spontaneous afferent discharge of subdiaphragmatic vagus nerve was recorded in 20% urethane-anesthetized rats. We investigated the effects of 5-HT and 5-HT3 receptor antagonist on the discharge of subdiaphragmatic vagus nerve by intravenous injection of different dosage of 5-HT(3, 10, 30μg·kg-1) and Granisetron (1mg·kg-1). 2. Spontaneous and gastric distention-induced afferent discharge of subdiaphragmatic vagus nerve was recorded in 20% urethane- anesthetized rats.We investigated the effects of endogenous 5-HT and 5-HT3 receptor antagonist on the discharge of subdiaphragmatic vagus nerve as well as the corresponding mechanism by duodenal infusion of hyperosmotic NaCl to evoke the excretion of 5-HT. 3.Spontaneous afferent discharge of subdiaphragmatic vagus nerve was recorded in 20% urethane-anesthetized rats.We investigated the effects of 5-HT and 5-HT+SEC on the discharge of...