Subcloning, Expressing, Purifing, And Primary Study Of F3T7's Biological Activity

The system of targeting antitumor drug to the tumor tissue is important for enhancing the curative effect of anti-tumor drugs and biological pharmaceuticals , reducing the dosage , especially lowering the side effect remarkably . So , We construct F3T7 clone vector pEKH-F3T7 .F3 peptide is a 31-aa peptide which is an N-terminal fragment of human high mobility group protein 2(HMGN2) .F3 peptide almost specificely binds to all the tumor cells and the vein endothelial cells , it also can carry a payload (phage , fluorescein) to a tumor . After intravenous inject with HMGN-2 , They can directionally distribute to the place where HL-60 and MDA-MB-435 tumors grew in the rabbit . This peptide may be suitable for targeting antitumor drugs to tumors .Tumstatin (the noncollagenous 1 domain of theα3 chain of typeⅣcollagen ) has the anti-angiogenic capacity , has been demonstrated to effectively inhibit angiogenesis and consequently the growth of solid cancer . The anti-angiogenic activity was localized to amino acids 54-132 , and the antitumor activity was localized to amino acid 185-203 .The 72-97 amino acids region of tumstatin named as Tum-7 was the smallest region which effectly inhibit the angiogenesis and cloned by us. The recombinatial...