| Vitamin E (VE) includes tocopherols and tocotrienols. VE is an essential micro-nutrition for the reproduction and growth of human being and animals. VE functions anti-oxide, anti-aging, anti-cancer, anti-hot stress, improving immune ability and muscle quality as well as influencing the transcription and expression of some genes, and so on. It is an important mission for nutritional physiologists to study the metabolism, control and application of VE.The postmitochondrial supernatant (S9) of liver was used in the present in vitro study since the S9 is rich in cytochrome P450 to catalyze VE to be metabolized. The aims of this study were: (1) to find methods to analyze 7-tocopherol and its metabolites;(2) to study the kinetics of 7-tocopherol metabolized in the S9 of male SD-rat liver in vitro;(3) to compare the differences of 7-tocopherol metabolized in the liver S9 of male SD-rat, male Brazil turtle and male cobia, respectively.To our knowledge, reverse phase of high performance liquid chromatography (RP-HPLC) was firstly developed to analyze the metabolites of 7-tocopherol with ideal results. Meanwhile, Gas chromatography/Mass chromatography (GC/MS) were used to analyze the structures of unknown metabolites of 7-tocopherol and a main unknown metabolite was identified as a side chain ω- hydroxylate of 7-tocopherol.The kinetic results of of 7-tocopherol reacted in the S9 of male SD-rat liver showed that the optimal time was 45 min; the optimal concentration of S9 5 mg/ml; the optimal concentration of NADPH 1 mM; and the optimal concentration of 7-tocopherol 8 μg/ml. The result of γ-tocopherol metabolized in the S9 of male SD-rat was the best; male Brazil turtle better; and male cobia worst.
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