Study Of Using Alkali-Insoluble Polysacchrides Of Auricularia As Support Materials Of Immobilized Cellulaes

It has been widely reported that fungal polysacchirides have bio-activities such as immunomodulatory, antitumor, antivirus and hypocholesterolemic effect and became a focus of biochemical research. They are also a kind of natural polymer materials due to their diverse molecular structures and well absorption affinities. Immobilized enzyme have both catalytic activities and reusable features and were extensively used in chemical industry, medical, pharmaceutical, biosensor etc. The preparation of alkali-insoluble polysaccharides from Aricularia auricula and cellulase immobilization were studied in this paper. The results may be beneficial to their industrial application.The soluble polysaccharides of Aricularia auricula(AAP) was extracted by the NaOH solution cooperated with ultrasonic treatment and the alkali-insoluble AAP was remained. The optimized extraction condition of insoluble AAP was: 0.1mol/L NaOH solution at a solid-to-liquid ratio of 1/100, ultrasonic treatment at a power of 160W. The largest amount of polysaccharides(49.5% in total weight of Aricularia auricula) was obtained at the condition of 0.12mm particle size below, extracting at 100℃for two hours.The cellulase was immobilized into the alkali-insoluble polysaccharides of Aricularia auricula. The immobilized cellulase by Cross-linking method (glutaraldehyde) has an activity of 49% to that of cellulase and showed good performance in recycling. Another support materials was alkali-insoluble polysaccharides modified by acrylic acid and it showed lower enzyme activities.The results in this paper are beneficial to the development of Aricularia auricula. The insoluble polysaccharides of Aricularia auricula is a new kind of immobilized enzyme support materials and needed to be further studied.