Cloning Of Expansin Gene In Chimonanthus Praecox And Construction Of Expression Vector

The expansin protein is a kind of cell wall zymoproteino This protein can break the hydrogen bond between the cell wall and multipolymer, and induce cell wall delay depended on acid and the relaxation pressure, it mainly codes by more than two kinds of genes families, named separately as α-expansin and P-expansin, each kind of expansin protein gene family also has the very many gene members. The expansin protein has been found in the different kind of plant, and a lot of expansin protein gene has been cloned. The research indicate that: each expansin protein gene expressed in the highly specific cell type and the highly specific position spot. Its expression is strictly controlled in the time and space, and influenced by the hormone and the environment factor. At present people thought the expansin protein participated in plant's nearly a whole growth process: besides it can increases the cell, the expansin has played a special role in the cell growing, the seed bourgeoning, the root hair forming, the root system growing, the leaf anlage forming, the leaf growing, the fruit maturing, the organ separated forming, as well as the pollen tube growing aspect.This experiment obtained the expansin for the first time from Calycanthus praecox's cDNA library, the name is Cpexpl,and has been carried on the sequence analysis and the function to it forecast, as well as construction of Cpexpl's expression vector. 1 .Get the expansin geneGet the expansin gene in library stochastic choice clone, distill DNA plasmid.take the library clone's plasmid as the template,using EST sequence DW222744 special primer we can get estimate...