Heat Shock Driven Gene Excision From Transgenic Plants And The Transformation Of Eucommia Ulmoides Oliv.

E. ulmoides Oliv., the specific plant in china, belongs to Eucommia and Eucommiaceae. It is not only a valuable medical but also the most important rubber plant in temperate zone.In this research we established the transformation system of E. ulmoides Oliv., studied the influence of ipt gene in plant transformation and growth by iransforming the Hsp_18.2:flp-35S:ipt into different plants, explored the heatshock condition and gene excision ratio of the gene heatshock excision system. Pave the way for the usage of this gene heatshock excision system in rare plants. The following are the results:1. Petunia axilaris and Nicotiana tabacum cv. Xanthin transformation with Hsp_18.2:flp-35S:ipt by Agrobacterium tumefaciensWe transformed Hsp_18.2:flp and Hsp_18.2:flp-35S:ipt into Petunia axilaris and Nicotiana tabacum cv. Xanthin; Studied the influence of ipt gene on regeneration and growth of Petunia axilaris and Nicotiana tabacum cv. Xanthin; Explored the heatshock condition and gene excision ratio of the gene heatshock excision system.Under the heatshock condition of 44 ℃ for 6 hours by 6 times, We aggregately heatshocked 309 transfgenic petunia plants and 191 Nicotiana tabacum cv. Xanthin, GUS activeity analyse showed 61 petunia plants and 42 Nicotiana tabacum cv. Xanthin have lost their GUS activeity. Among them the phenotype of ipt06-01 and ipt11-03 have changed obviously and recovered to the wildtype.The result showed that, ipt gene could efficiently promote the regeneration of Petunia axilaris and Nicotiana tabacum cv. Xanthin in transformaiion. And petunia plants transformed with ipt gene could outshoot lots of branches which provided much facility in its multiplication. But the ipt gene could also take some...