Studies On Gene Transfer Making Tube Embryo In Porcine By Using Spermatozoa As Vector

In this paper, four experiments were conducted to study the porcine in vitro fertilization and used sperm as vector for transmitting. In order to establish technology platform of sperm-mediated gene transfer for porcine, which can improve the efficiency of fertilization and spermatozoa transferction in vitro, as well can obtain bioreactor of mammary gland in transgenic pig, we have carried out the studies on the fertilization and spermatozoa transfection of pig oocytes and spermatozoa in vitro. The main results of experiments as following:Section 1 The objective of this part is to study the factors that affecting in vitro fertilization of porcine. Our study divides into 5 independent tests, which comprise of adding different proportion of porcine follicular fluid into the maturation medium; different maturation medium and different temperature of IVM ;utilizing different methods of pig oocyte maturation in vitro and different methods of collection oocytes (aspiration ,dissection .mechanical method) separately.Section 2 The objective of this part is to study the effects of sexual maturity on IVM and IVF. The results indicate that: (1) maturation rate of different stages of sexual maturation haven' t significantly difference, but the sexual maturated porcine had a higher cleavage rate than not sexual maturated pig . (2) prolonging the maturation time in vitro of sexual unmaturated porcine' s oocytes could enhance cleavege rate to some extent , but that haven' t different performances in maturation rate. (3) prolonging the maturation time in vitro of sexual maturated porcine' s oocytes have lower cleavege rate than maturation 44h in vitro. (4) higher concentration of FSH and LH could enhance the maturation rate.