Cloning And Functional Analysis Of Cellulose Synthase Gene CDNA Of Ramie (Boehmeria Nivea)

Ramie is one of the most important economic crops in China, its cortex fibers are the main product that to be harvested. Recent researches on molecular mechanism of cellulose biosynthesis in some model plants have made great progress and providing perfect alternative information for understanding the molecular mechanism of cellulose biosynthesis in ramie.In this research, ramie was used as material, the ramie cellulose synthase gene cDNA most sequence was cloned by combination the techniques of degenerate PCR and RACE. There are only 450bp of the 5'very end of the expected cDNA still under pursuing. The cloned cDNA for cellulose synthase is designated as BnCesA1. Sequencing analysis showed the cDNA sequence be cloned is 3276 bp with a partial ORF from neuclotide 1 to 2814. Bioinformatic analysis ascertained this cDNA sequence is the ramie cellulose synthase gene and was submitted to GenBank. Its accession number is DQ077190.In order to investigate the expression model and regulation mechanism of BnCesA1 in different tissues of ramie, the nonconservative sequence at 3'terminal of BnCesA1 was amplified by semi-quantitative RT-PCR respectively at the same time. Taking 18S rRNA gene as an inner control, the integrate optic density (IOD) of BnCesA1 and 18S rRNA gene bands were detected with gel analysis software and defined the ratio of IOD as relative expressive quantity. The results indicated that BnCesA1 expressing both in leaf, stem, root and bud. The expression level from high to low is stem, leaf, bud and root, the relative content is: 0.791, 0.381, 0.319, 0.183 respectively. The expression data presented shows that BnCesA1 is highly expressed in developing stem undergoing significant secondary cell-wall biosynthesis as well as...