Prokaryotic Expression Of Listeria Monocytogenes Hly And ActA Genes And Development Of Monoclonal Antibodies Against Their Products

Listeria monocytogenes is a gram-positive, facultatively intracellular bacteria and is also a food-borne pathogen for animal and hunman's listeriosis. The virulence of Listeria monocytogenes depends on its capacity of invading and multiplying in professional phagocytes as well as in normally nonphagocytic host cells. A number of virulence factors involved in the intracellular life cycle of Listeria monocytogenes have been characterized. Listeriolysin O and ActA protein are essential for Listeria monocytogenes infection. In this study, recombinant bacteria expressing hly and actA gene of Listeria monocytogenes were constituted respectively; the expressed LLO and ActA proteins bearing their immunobiological characteristics were acquired and hybridoma cell lines secreting McAbs against LLO and ActA were generated. All these results would bu useful for studying the pathogenicity and the immune mechanism of Listeria monocytogenes, and for developing the method and technique in the detection of Listeria monocytogenes.1. Prokaryotic expression of Listeria monocytogenes hly and actA genes in recombinant E.coliListeriolysin O and ActA protein, the products of hly and actA gene, respectively, are indispensable for Listeria monocytogenes to establish a productive intracellular infection. LLO is a secreted protein, which is essential for vacular lysis, making Listeria monocytogenes replicate in the host cytosol; ActA is a surface protein, which mediates...