Genetic Analysis Of Resistances To Aluminum And Salt In CED-9 Transgenic Plants

Ced-9 is a Bcl-2 analog from Caenorhabdities elegans that promotes cell survival. In this paper, we transferred CED-9 gene into tobacco and Arabidopsis by Agrobacterium tumefaciens EHA105, and investigated the function of the CED-9 when the plants were under Aluminum and NaCl stresses. The results are showed as followes:1. We constructed the expression vector pER8-ced9, and transferred CED-9 gene into Arabidopsis by vacuum filtration. The work of using hygromycin selective medium to obtain regenerated plants is still going.2. Using recombination techniques, the gene fragment CED-9, derived from pBS, was incorporated into pGEM-7zf(+) and formed a new vector pGEM-ced9, in order to get the right endonuclease sites. And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. The CED-9 gene was transformed into leaves of tobacco by Agrobacterium tumefaciens EHA105. PCR analysis and Southern blotting, confirmed that the CED-9 gene has been integrated into the chromosomal DNA of these transgenic plants.3. T1 seeds were harvested and then used to genetic analysis after selection in the kanamycin cuture. We obtained 4 transgenic lines with segregation of 3:1,and confirmed 2 lines expressing CED-9 by RT-PCR.4. The transgenic tobacco plants expressing CED-9 showed strong resistance to aluminum toxity and salt stress compared with wild plants. It was suggested that CED-9 conferred enhanced resistance to cell death induced by abiotic treatment and affected plant growth and development.