The Application Of Biological Mass Spectrometry In Studies Of Activator Protein From Fungi

A proteomics approach has been apply to investigate a new kind of protein fungi elicitor, which named Activator. These protein elicitors are from different types of fungi including: Alternaria sp., Rhizoctonia solani, Aspergillus sp., Botrytis sp., Magnaporthe grisea, Penicillium sp., Trichoderma sp., Fusarium sp. et al. Activitors from Alternaria, Botrytis and Magnaporthe have be indentified by the MALDI-TOF, MALDI-TOF/TOF, nanoLC-ESI-IT-MS/MS system. PMF, de novo sequencing and MS/MS techology have been usd to study the protein. Activitor from Alternaria has been measured the molecular mass by microLC-ESI system.The result shows that the activor are a new kind of protein, which haven't be reported before. Part of it's sequence are homology to alpha-NAC protein in other fungi, which play an importein role in preventing nascent polypeptides from binding inappropriately to membrane-targeting apparatus during translation, but is also active as a transcription regulator.PTM analysis found that the activator from Botrytis was ubiquitination. What the function of this modification is unkown.Using Neurospora crassa as model fungi, the proteome of fungi have be studied. We used liner gradient SDS-PAGE-nanoLC-ESI-IT-MS/MS system indentified 1467 proteins. This system can be used to study Activor-plant interaction at the proteomics level.