| With the development of molecule biology the improvement of insect baculovirus expression vector systems (BEVS), it had become an efficiency approach to express active proteins and bacterins. The genomes of many kinds of baculovirus were already sequenced. As the Antheraea pernyi baculovirus expression vector system has many particular virtues,such as higher efficiency of promoter, higher expressions and lower cost , it is necessary for us to investigate the genome of Antheraea pernyi nucleopolyhedrovirus (ApNPV). we established the genomic library of radome plasmid. Results of Measuring and megaligning the inserted sequence, three new genes were obtained and analyzed.1 Genes structure were as follows : the odv-e56 gene which coded the occlusion-derived virus envelope fusion protein had a late gene motif TTAAG, its ORF had 1125 bp and encodes 374 amino acides, there were six conserved cysteine residues, a glycosylation site and two hydrophobic domains; pe-38 and ie-2 are immediate early regulator genes which their ORFs encoded 286 and 302 amino acides separately, they also had a conserved zinc finger and a leucine domain separately.2 By comparing the identities of nucleotide acid sequences and amino acid sequences with the other baculoviruses, we found that the ApNPV gene odv-e56 was highest homological with that of Orgyia pseudotsugata nuc1eopolyhedrovirus (OpNPV), but lowest homological with that of Neodiprion lecontei nucleopolyhedrovirus (NlNPV). ApNPV pe-38 and ie-2 gene were the highest homological with that of OpNPV,but lower than that of Bombyx mori nucleopolyhedrovirus (BmNPV).3 From the evolution of the nucleopolyhedrovirus, we concluded that the evolution of ApNPV odv-e56 had two kinds of methods: one was point mutation and the other was short of amino sequence; the conservetaive domain of pe-38 and ie-2 were little and more amino acids are shorted, so they were typical genes of investigating evolution and relationship in the...
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