Study On Interaction Between ICT Fluorescence Probe And Bio-macromolecules

Chapter 1: The principle and the development of intramolecular charge transfer fluorescence were described. Recent developments of the HSA and DNA interactions with Spectroscopic probe were been reviewed.Chapter 2: The spectrum and photophysical properties of a new intramolecular charge transfer (ICT) probe namely 4'-dimethylamino-2,5-dihydroxychalcone (DMADHC) were studied in different solvents by using steady-state absorption and emission spectroscopy. Whereas the absorption spectrum undergoes minor change with increasing polarity of the solvents, the fluorescence spectrum experiences a distinct bathochromic shift in the band position and the fluorescence quantum yield increases reaching a maximum before decrease with increasing the solvent polarity. The magnitude of change in the dipole moment was calculated based on the Lippert-Mataga equation. These results give the evidence about the intramolecular charge transfer (ICT) character in the emitting singlet state of this compound.Chapter 3: The photophysical properties of a new compound 1-Keto-2-(p-Dimethylaminobenzal)-Tetrohydronaphthalene in various solvents at room temperature were characterized by the absorption and steady-state fluorescence technique. The bathochromic shift on the emisson spectra, the broad halfwidth of the fluorescence band and the increase in the excited state dipole moment occurred. These gave the evidence about the intramolecular charge transfer (ICT) character in the emitting singlet state of the compound.Chapter 4: 4'-dimethylamino-2,5-dihydroxychalcone (DMADHC) exhibited dramatic enhancement of fluorescence intensity with accompanying blue-shift of the emission maximum when the concentration of human serum albumin (HSA) was increased. Binding to HSA also caused a progressive...