Tissue Culture Of Five Woody Plants And Study Of Physiological Biochemistry

This　paper　studied　the　plants　regeneration　under　various　treatments　by　taking　the　stem　tips　of　Albizzia　julibrissin,Laburnum　anagyroides,Sorbus　aucuparia,Cerasus　humilis,Calluna　vulgaris　as　explants.　And　during　in　vitro　tissue　culture　of　Albizzia　julibrissin,Laburnum　anagyroides,　the　relationship　between　the　morphosis　and　the　variation　of　primary　physiological　index　was　studied　for　a　base　of　efficient　controlling　in　vitro　　organic　morphosis　.　The　results　showed　that:　　adventitious　shoots　of　five　woody　plants　were　induced　on　MS　+　6-BA　0.5～1.0　mg/L+　NAA　0.1　mg/L　and　the　differentiation　rate　was　higher.　At　the　same　time,　the　effect　of　the　auxin,　the　sugar　density　and　the　culture　medium　on　plantlet　rooting　was　studied　for　the　optimal　medium　on　rooting　,　which　was　1/4MS　+　IBA　0.5　mg/L+　sugar15g/L.So　the　efficient　plant　regeneration　system　was　established.　The　concentrations　of　primary　physiological　index　in　the　differentiation　of　Albizzia　julibrissin,Laburnum　anagyroides　stem　tips　were　studied,　which　rise　in　the　prophase　of　formation　and　drop　later.　These　results　demonstrated　that　　in　woody　plants　there　were　some　relationships　between　the　morphosis　of　adventitious　shoots　and　the　concentration　of　POD,soluble　sugar　and　soluble　protein　.